1. Which of the following statements about glycolysis is correct?

A) Produces pyruvate

C) Occurs in the cytoplasm

B) Can occur anaerobically

D) All of the above

2. Arrange the following intermediates of glycolysis in sequence:

A) Glucose → G-6-P → F-1,6-BP → PEP→  Pyruvate

C) Glucose → F-6-P → F-1,6-BP → PEP → Pyruvate

B) Glucose → G-6-P → F-1,6-BP → Pyruvate → PEP

D) G-6-P → Glucose → F-1,6-BP → PEP → Pyruvate

3. The step in glycolysis where ATP is consumed involves the enzyme:

A) Aldolase

C) Hexokinase

B) Enolase

D) Pyruvate kinase

4. How many ATP molecules are consumed during the energy investment phase?

A) 0

C) 1

B) 2

D) 4

5. What is the net gain of ATP molecules in glycolysis per glucose molecule?

A) 0

C) 2

B) 1

D) 4

6. What is the first irreversible step in glycolysis?

A) G → G-6-P

C) F-6-P → F-1,6-BP

B) PEP → Pyruvate

D) 1,3-BPG → 3-PGA

7. The total number of enzymatic steps in glycolysis is:

A) 8

C) 9

B) 10

D) 12

8. Which step of glycolysis is regulated by feedback inhibition by ATP?

A) G → G-6-P

 C) F-6-P → F-1,6-BP

B) PEP → Pyruvate

D) G3P → 1,3-BPG

9. The conversion of phosphoenolpyruvate to pyruvate generates:

A) NADH

C) FADH2

B) ATP

D) H2O

10. During glycolysis, how many NADH molecules are produced per molecule of glucose?

A) 1

C) 2

B) 4

D) 6

11. The glycolytic pathway is common to both:

A) Aerobic and anaerobic respiration

C) Photosynthesis and respiration

B) Gluconeogenesis and fermentation

D) Oxidative phosphorylation and Krebs cycle